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A Method for the Detection and Control of Leptospira Infection in Humans

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<b>Features</b> :
* e-Auction Description of Work : A Method for the Detection and Control of Leptospira Infection in Humans
* e-Auction Type : NonExclusive
* e-Auction Number : NE/KIHT/20060
* TRL : 5
* Base Price : 400000.00
* e-Auction Date / Time : 30-11-2018 16:02

* Leptospirosis is emerging and re-emerging disease affecting humans, animals and environment causing socio-economic loss as well as threat to lives. Though more than 500,000 severe human cases are estimated globally, leptospirosis is still considered as neglected tropical disease and in India, lacks awareness because of unreported data. However, there has been increasing numbers of reported outbreaks of leptospirosis from Kerala, Gujarat, Tamil Nadu, Karnataka and other parts of India. Based on extensive research, we have identified a stretch of unique nucleotide sequence in Leptospira, which can be amplified using polymerase chain reaction (and its variants) to detect even low levels of the parasite. The diagnostic method can be used to differentiate between species of the parasite.
* The method has been tested with infected and with enriched samples, with known numbers of Leptospira nucleotide polymers. The amplification method with specific primers detected the parasite levels up to 0.1 copy per 10 ¼l of blood sample
Need & Demand :
* There has been increasing numbers of reported outbreaks of leptospirosis from Kerala, Gujarat, Tamil Nadu, Karnataka and other parts of India. The warm and humid climatic condition of Southern India favors the growth and survival of its causative agent, Leptospira species
* In symptomatic and asymptomatic infections, a sensitive, accurate and rapid diagnostic tool is essential to treat leptospirosis
* The method is highly sensitive and capable of detecting up to 0.1 copy of the parasite DNA per 10 ¼l in human blood
Market Access Information :
* The current market for Leptospira diagnosis is reliant on kit-based methods that rely on agglutination or enzyme immunosorption or antibody-antigen interaction
* All the above tests have several drawbacks in terms of reliability, sensitivity etc. paving the way for novel diagnostic methods
* Nucleotide-based amplification method has potential to occupy the market space
Future Developments :
* Early and rapid diagnostic tests, based on identification of specific nucleotides of an organism is the trend and is likely to develop in scale due to the inherent specificity and sensitivity associated with such technique. This current method developed exhibits improved sensitivity in detection and is a rapid method too.
* Requires optimization of kits

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A Method for the Detection and Control of Leptospira Infection in Humans

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